The mycelial mass coming from aerobically grown cultures was
added into the anaerobic fermentation media, which was the apple
pomace hydrolysate. 40 mL hydrolysate was added into 50-mL
Erlenmeyer flasks in order to leave ~20% of the culture flask volume
as air space. Fermentation experiments were conducted for 5
days at 30 C. Samples were taken within certain time intervals,
centrifuged at 6000 g for 15 min. The supernatants were stored
at 18 C for further analysis.