2.2.5. Chlorophyll
Chlorophyll content in chillies was measured following the method. Ten grams sample was taken and extracted with acetone in a blender. The extractionwas repeated with acetone till the residue was colourless. The filtered contents were taken in a separating funnel along with50 ml of diethyl ether. Water was added 5–10 times to remove the acetone layer until the ether layer is free of acetone. The ether extract was transferred to a 100 ml volumetric flask and made up the volume with ether. Anhydrous Na2SO4 was added to remove the traces of moisture.