The induced cells were harvested by centrifugation (8000 g,15 min, 4 C), resuspended in phosphate buffer (50 mM, pH 7) containing 300 mM NaCl and disrupted by French Press passages (three times at 1100 psi).
The induced cells were harvested by centrifugation (8000 g,15 min, 4 C), resuspended in phosphate buffer (50 mM, pH 7)containing 300 mM NaCl and disrupted by French Press passages (three times at 1100 psi).