The total GSH contents of the cell lysates were estimated by
colorimetric assay using the reaction between sulphydryl group of
DTNB to produce a yellow-color of 5-thio-2-nitrobenzoic acid (TNB).
TNB production is directly proportional to the concentration
glutathione in cell lysates. All experiments were done in triplicate.
Total glutathione was determined by measuring the absorbance
410 nm after 30 min incubation. The results were expressed in
µmole per mg protein.