Fig. 1. (A) Representative melting peaks (˚C) for Schistosoma japonicum (a), Schistosoma mekongi (b), mixed-plasmids (c), and distilled water (d). Amplification plot of fluorescence vs cycle number showing analytical sensitivity of HRM real-time PCR for detection of S. japonicum (B) and S. mekongi (C) plasmids: e-j; 10-fold serial dilutions of S. japonicum or S. mekongi plasmids, from 4.3×107 to 4.3 ×102 copies per reaction. k; distilled water (negative control).