Eucalyptus production is of great importance to Brazil’s economy. Diseases affecting the growth and the architecture of
these trees lead to large losses in yield and quality of the wood. The occurrence of eucalyptus trees showing witches’
broom and little leaf symptoms in different areas of Brazil is affecting seedling production. In this work, a phytoplasma
was found associated with these symptomatic plants and was identified as belonging to the 16SrII group. This is the first
report of a phytoplasma associated with eucalyptus in Brazil.