Two strains , Rubrivivax gelatinosus SB24 and Rhodobacter sphaeroides SB46/1, isolated in Thailand, had high efficiency in production of hydrogen, a clean fuel, with either starch of malic acid. The bacteria produced only H₂ and CO₂ and the ratio of H₂ was extremely high 80-95%. Capability in producing high percentage of H₂ leaded to high potential to combine the biohydrogen production with fuel cell. The research collaboration between biological production of h2 and hydrogen fuel cell would become plausible, The collaboration between the 2 projects has been investigated and leaded to be granted by Associate Professor Dr. Terdthai Vatanatham, Department of Chemical Engineering, Faculty of Engineering, Kasetsart University. This paper presented the initial rate of H₂ production of the 2 phototrophic bacteria showed possibility to further imply into large scale production of H₂. By initial rate of H₂ production, the 2 Genus, Rubrivivax and Rhodobacter, preferred different electron donors. Rubrivivax gelatinosus SB24, preferred starch to malic acid. Either raw or cooked starch of cassava, rice, sticky rice or mungbean was food with initial rate of h₂ production of 8-25 mLh2/L culture/h while that with malic acid was only 3 mLH₂ /L culture/h. Rice and sticky rice were the best. Efficiency in utilization of raw starch was high (85-99%). On the otherhand, Rhodobacter sphaeroides SB46/1, evolved high H₂ with malic acid but almost none with all kinds of starch at the initial time. It's initial rate of H₂ production with malic acid was 20 mLH₂ /L culture/h. WE were successful in production of hydrogen in 6 litre-designed photobioreactor. Rubrivivax gelatinosus SB24 produced promising amount of hydrogen. Wastewater of cassava could also be used by Rubrivivas gelatinosus SB24 to production H₂. With the present experiment in 60mL culture, final concentration of the waste water up to 18% was recommended. The higher the concentration was, the lower the H₂ produced. The process also resulted in decreasing COD and starch in the wastewater to 33 and 78% respectively.