was used: [(Etarget)DCt,target(controlsample)] [(Eref)DCt,ref(controlsample)],
where E is the primer efficiency in the reaction, target is the gene of
interest, ref is the reference gene and DCt is the difference in cycle
number between control and sample when they pass a certain fluorescence
threshold. A standard curve was made for each primer pair
to calculate the efficiency of the primer in the reaction. All primer sequences are shown in Table S2.