Also, few studies have been conducted in production
of protein-loaded GNP by this method. In the present
study, a two-step desolvation method was used to
produce GNP containing a model protein drug, BSA,
as protein/peptide delivery system. At the first step, the
influence of molecular weight heterogeneity of gelatin
on nanoparticle size and PDI was investigated. Then,
BSA-loaded GNP was produced and the optimum
amount of BSA loading was obtained. Finally, the size,
shape, entrapment efficiency (EE%) and drug