2.3 Mushroom harvesting
After incubation period, the bags were transferred to a cultivation chamber at 25 ± 2 ºC and 90% relative air
humidity in the presence of light throughout the entire harvesting period. The mushrooms were harvested at
maximum development but with the hat closed. The mushrooms were weighed to determine their biological
efficiency (BE), which was calculated with the following equation: BE = (fresh mass of mushroom / dry mass of
substrate) x 100.