For the count of total mesophilic aerobic bacteria
(TMAB), Plate Count Agar (Merck 1. 05463) was
inoculated by using pour plate method and reproduced
colonies were counted after 48 hours of incubation at 37 °C
[6]. Violet Red Bile Agar (Oxoid CM107) was used for the
purposes of isolating coliform bacteria. Red violet colored
colonies of 2-3 mm diameter were counted after 24 hours
of incubation at 37 °C, which was realized after inoculation
with double layer pour plate inoculation method [7].