Electrophoresis was performed at 60 C for
13 h at a constant voltage of 100 V. Following electrophoresis, the
gel was stained with SYBR Gold nucleic acid stain (Invitrogen, USA)
for 30 min. The images were visualized on a UV transilluminator
and captured using Biovision CN 1000/26 M (Vilber Lourmat,
France).