Samples were withdrawn every week and analyzed for
residual ethanol4 and acetic acid by the distillation method
of Amerine et al.1 The latter were correlated with acetic
acid analysis using a gas liquid chromatograph (Nucon,
India make) with a PoraPack-N column (mesh range 80–
100, internal diameter 2 mm and column length of 2 m)
and under conditions of injector, oven and detector temperatures
of 200, 75–200 and 200°C respectively. For gas
chromatography, samples were clarified through charcoal
and 10 μL samples were injected.