The weight average (Mw) of the polysaccharides was determined by GPC–ELSD (gel permeation chromatography–evaporative
light scattering detector) using a TSKgel GMPWXL (7.8 mm * 300 mm) column.
A mobile phase of 0.1 mol/L NH4OAc solution filtered on 0.22 lm filters was used.
The flow rate was 0.5 ml/min and analyses were performed at 30 C. 5 mg sample was resuspended in 1 ml distilled water.
This mixture was allowedto solubilize briefly and then filtered on 0.22 lm filters.
The gel permeation column was calibrated using polyethylene glycol/polyethylene oxide standards.
The data was processed by WinGPC UniChrom GPC Software, made by Polymer Standards Service (PSS).