A modified method described by Hanny and Shizuko [35] was used for biodiesel production that is twostep transesterification reaction. The composition of the FAME was analysed with gas chromatograph–mass spectrometry (GC–MS). The sample was analysed on a Shimadzu GC–MS system model QP 2010 PLUS equipped with a quadrapole mass analyser.
The GC column was a medium polarity capillary column (BPX-5column (29.4 m 0.25 mm), with film thickness of 0.25 lm) withhelium as the carrier gas.