abstract
The dynamic of Saccharomyces cerevisiae, Pichia kluyveri and Hanseniaspora uvarum during spontaneous
and inoculated cocoa fermentations and their effect on sensory characteristics of chocolate were
investigated. Yeast populations were assessed by qPCR. S. cerevisiae was predominant during spontaneous
(average 5.4 log cell/g) and inoculated (average 7.2 log cell/g) fermentations. The H. uvarum
seemed to be suppressed by the other two yeasts, as it showed similar population (approximately
4.0 log cell/g) even in the inoculated assay. Carbohydrates were consumed quickly at inoculated
fermentation (68% and 42% were consumed in the inoculated and control assays respectively, at 24 h).
Ethanol content was higher in the inoculated (8.3 g/kg at 48 h) than in the control (4.6 g/kg at 96 h)
fermentation. Consumers did not report a significant preference for either chocolate (p < 0.5). However,
differences in the flavor attributes were noted, as consumers reported stronger coffee and sour attributes
in the inoculated assay. This is the first time qPCR has been used to assess the dynamic of yeasts during
the complex fermentation of cocoa beans. The inoculation accelerated the process. S. cerevisiae and
P. kluyveri likely contributed coffee, sour and bitter flavors to the inoculated chocolate.
abstractThe dynamic of Saccharomyces cerevisiae, Pichia kluyveri and Hanseniaspora uvarum during spontaneousand inoculated cocoa fermentations and their effect on sensory characteristics of chocolate wereinvestigated. Yeast populations were assessed by qPCR. S. cerevisiae was predominant during spontaneous(average 5.4 log cell/g) and inoculated (average 7.2 log cell/g) fermentations. The H. uvarumseemed to be suppressed by the other two yeasts, as it showed similar population (approximately4.0 log cell/g) even in the inoculated assay. Carbohydrates were consumed quickly at inoculatedfermentation (68% and 42% were consumed in the inoculated and control assays respectively, at 24 h).Ethanol content was higher in the inoculated (8.3 g/kg at 48 h) than in the control (4.6 g/kg at 96 h)fermentation. Consumers did not report a significant preference for either chocolate (p < 0.5). However,differences in the flavor attributes were noted, as consumers reported stronger coffee and sour attributesin the inoculated assay. This is the first time qPCR has been used to assess the dynamic of yeasts duringthe complex fermentation of cocoa beans. The inoculation accelerated the process. S. cerevisiae andP. kluyveri likely contributed coffee, sour and bitter flavors to the inoculated chocolate.
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