In vitromicrotuber formation potentiality of potato was investigated to establish a rapid disease free seed
production system in potato. MS medium supplemented with 4 mg/L of KIN showed best performance in
respect of multiple shoot regeneration and microtuber formation. Simple MS medium was not able to produce
any micro tuber under in vitrocondition. Dark condition better responded to tuberization than light condition.
Among the three different explants (nodal segment, sprout and shoot apex) nodal cutting showed the best
performance on days to microtuber formation and average weight of microtuber. MS + 6% sucrose + 4 mg/L
KIN combination of treatment was best for in vitro tuberization among the parameters under study.