reducing the spore germination rate to 14%, while the water control germination rate reached as high as 97%. B. cinerea spores were sensitive to the chitosan film incorporating 0.1% GSE or 0.5% GSE alone. More than 60% of the spores germinated in PDA plates after a 60 s immersion in 1% chitosan alone. The combination of 0.1% GSE and 1% chitosan achieved a significant result, reducing the spore germination rate to only 25%, indicating an apparent combined antifungal effect of chi- tosan and GSE. For comparison, the antifungal activity of 0.1% TBZ on gray mold was also examined. According to the results in Fig. 1, 29% of the spores germinated after 60 s immersion in 0.1% TBZ. Thus, the GSE or GSE + chitosan treatment could inhibit spore germination of B. cinerea to a greater extent than 0.1% TBZ.