WT
(Fig. S5). However, the growth defects of 3C2 and 3D6 do not
correlate to the degree of Thaps3_264297 knockdown (Fig. S3 B
and C), indicating that secondary effects of integration, expression,
or processing of the RNAi construct may be occurring in these
strains—a phenomenon found in plant transformations (29). We
also investigated the growth of 1A6 and 1B1 under a 12 h:12 h light:
dark regime for 116 h. Strains 1A6 and 1B1 grew comparably to
WT under light:dark conditions (Fig. 2B), demonstrating similar
growth rates to WT during both the light and dark periods (Fig. 2C)