a All spectra were acquired using elecfrosprav ionizafion conditions Each entry in the table represents a separate acquisition of the data.
The calumn labeled “No.” references the posifion of each peptide to Figure 3. The column designation Db refers to the specific species
database used in the search. E-E CO/I; H = human, Y= veest. The column labeled “Protein identified” hsts the protein from which the
peptide was derived. Under the heading “Sequence identified.” the amino acid sequence represented by the tandem mass spectrum is
listed. The column designation AC, refers to difkrence of the correlation parameters for the first- and second-ranked sequence8 from the
species-specific database search. The rankings of the correct sequence following a search of the species-specific and Genpept databases,
es determined by inspection of the tandem mass spectrum, we listed in the columns designated by the letters S and G. respectively. The
mess tolerance used in the search was *0.05% or a minimum of f 1 u Fragment ion mass tolerance we5 f 1 u_ Sequences marked with
en astensk used a mass for Lye mcreased by 42 u. All tandem mass spectra were acquwed on doubly charged ions with the exceplion of
number 49, which was triply charged. The abbreviation SC refers to S. cerevisiae