2.4. Cellulase production
Small scale experiments were carried out in metal
shallow trays (50x40x5 cm). After inoculation with
10% liquid seeds, the trays were installed in an incubator
in which air humidity and temperature were kept at
92% and 30°C, respectively.
Large scale production of cellulase was performed in
a deep trough fermentor (4x2x1.5 m), the thickness
of the solid substrate layer was 30cm and the temperature
was controlled at 28–30°C, air with over 90%
humidity blew through the bottom of the cultivation
chamber by the forced aeration.