Abstract Scarcity of pure water is a threatening issue worldwide; water is an essential need for human survival and all activities on earth. Effluent water from industries containing recalcitrant pollutants causes dangerous impacts to the envi- ronment and human health. In the current epoch, bioremediation is an alternative technology for decontamination of water systems by use of specific microorganisms and it can provide green, efficient, cost-effective, and sustainable remediation of water contaminants. Immobilized nanofibers possess enhanced catalytic activity, high stability, and very good reusability of novel nano-biocomposites which has remarkable potential for the treatment of water and wastewater. It also plays a major role in safe preservation of bioremediating bacteria for potential wastewater treat- ment applications. Nanofibers have become a popular carrier matrix for immobi- lization of specific microorganisms. Simple, versatile, and cost-effective properties of nanofibers made them a promising tool for microbial integration which enhances the bioremediation by efficient removal of contaminants such as dyes and heavy metals from wastewater. This chapter describes the immobilization of specific bacteria on electrospinning nanofibers and its application in bioremediation process.