Samples from various points in the opiate alkaloid extraction process were analysed using this approach and the results
were compared to those of a standard GlaxoSmithKline method based on ion pairing HPLC and UV-absorbance detection.
The samples were analysed on site immediately after collection to minimise degradation.
Dilution of the samples was required to operate within the linear calibration range, due to the sensitivity of tris(2,2-bipyridyl)ruthenium(III) chemiluminescence reagent.