Materials and methods: We interviewed 14 key informants and 438 non-specialist informants about their traditional knowledge of medicinal plants. We tested normality of the data and correlations with distance to the nearest city using Kolmogorov–Smirnov tests and Spearman's rank correlation coefficient. Cluster analysis and cultural importance index (CI) were calculated for the similarity of medicinal plant used and culturally importance medicinal plant species among Karen villages respectively.