For the in vivo study, thirty
fish were placed into 3 aquaria and each group received
a diet consisting of a non-supplemented commercial diet
(Skretting) (control group) or the same diet supplemented
with 0.5% or 1% of inulin. To prepare the diets, a commercial
pellet diet was crushed, mixed with the appropriate inulin
concentration and water, and made again into pellets, which
were allowed to dry and stored at 4 C until use. Control
diets were prepared adding only water.