During bioreactor fed-batch cultivations in a glycerol-based medium, accumlation of 3-HPA was successfully decreased, leading to enhanced 1,3-PD synthesis (35.6 vs. 25.5 g/L), at higher yield (0.49 vs. 0.44 mol/mol at 48 h) and productivity (12.41 vs. 8.06 g /g and 0.32 vs. 0.23 g/(L*h)).