The study focused on the influence an increased copper content in the M and SKooG(1962) medium on the and development of Dendrobium kingia num Bidwill. solid were used for of orchid plants on/DIH Sterile plants of pseudobulbs NAA and 1.0 mg dm kinetin. 0.5 mg dm3 the MS regeneration medium supplemented with Cas cuso, 5H o was added to all the tions in concentrations of 0.025 2.5 trol, 0.625, 1.25, 2.5 and 5.0 mg dm The results showed that the treatments with 1.25 and stimulated the orchid growth and development in n vitro culture. After eight months of growing in in vitro culture, the bighest number of shoots, the longest roots and the heaviest fresh weight of plantlets were obtained in these treatments. In medium with the highest copper concentration(5.0 mg dm3. a negative influence of the metal on the length of roots and fresh weight of orchids was noted. Spectrophotometric analysis(ASA) showed that the copper and iron accumulation increased in both shoots and roots with the increase in the extern Cu level, whereas the zinc and calcium accumulation in these organs decreased. The copper and zinc ac cumulation in the roots was about 1.5-2.5 times higher than in the shoots, but the iron accumu lation was about 3-3.5 times higher. The calcium accumulation in roots was only 5-12% higher than in shoots