recent research studies have suggested that fish are capable of The sensory or pain perception. These investigations have demonstrated bony fish have that The sensory receptors to detect potentially painful stimuli (Fig 1), which are very similar to those found in mammals A variety of species also exhibit exhibit adverse behavioral and physiological responses to a potentially painful event that are ameliorated by the use of analgesia (Fig2) therefore it would seem prudent to reduce any pain and discomfort during surgical interventions or when tissue damage has or is likely to occur. Anesthetic drugs have historically been used on fish in aquaculture, experimentation, and veterinary practice. Their use reduces the stressful impacts of handling, especially during routine procedures such a weighing, vaccination, blood sampling tagging, experimental surgery, and veterinary procedures. therefore light anesthesia or sedation can improve the efficiency of rapid procedures. In invasive procedures in which tissue damage will occur full or deep anesthesia or loss of consciousness is used to improve fish welfare and minimize the pain associated with surgical intervention. Anesthetic administration is usually achieved via immersion with the drug being dissolved in water where the fish is held or an anesthetic vessel such as a tank or large bucket (also termed inhalation). When induction takes place through immersion, it is paramount to observe the fish and gauge the depth of anesthesia to reduce the incidence of overdose. Uptake of these chemicals is primarily via the gills and possibly the skin. The level of anesthesia is monitored tored by recording gill ventilation rates, maintenance of equilibrium (upright position), and reflex responses (e.g., swimming respons to tail pinch).Research studies have measured rates of induction and recovery, responsiveness to external stimuli and handling, as well as pharmacokinetics of these agents. However,these research investigations often focus on a small range of species, testing only one anesthetic agent or a range of drugs on one species.