FIG. 1. Yolk protein concentrations by reproductive stage. The female Sicyonia ingentis were staged and hemolymph concentrations determined using the ELISA. The Vg levels significantly rise during stage 2–4, where it peaks before significantly decreasing in stage 5, which immediately precedes ovoposition. Means (±SEM) followed by the same letter are not significantly different (1-way ANOVA, Tukey's Test @ P < 0.05). From Tsukimura et al., 2000