The biochemical oxygen demand (BOD) test is a crucial environmental index to determine the relative oxygen requirements of wastewaters, effluents and polluted waters. It measures the molecular oxygen utilized during a specified incubation period for the biochemical degradation of organic material (carbonaceous demand) and the oxygen used to oxidize inorganic material such as sulphides and ferrous ions. It can also be a measure of oxygen used to oxidize reduced forms of nitrogen (nitrogenous demand), unless their oxidation is prevented by an inhibitor. The conventional BOD test requires a five day incubation period at 20 °C and demands skill in determination, thereby, making it unsuitable for process control. Thus, it is necessary to develop a measurement method that could circumvent the weaknesses of the conventional method. The fast, portable and cost effective methods for environmental monitoring has stimulated the development of a variety of field analytical tools such as biosensors. Biosensors are devices that transduce a selective biochemical response to a measurable signal. Several biosensor methods for BOD measurement have been developed