Total antioxidant capacity using phosphomolybdate method: 0.3 mL of wine sample was mixed with 3 mL reagent solution
(6 M sulfuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate. The
reaction mixture was incubated at 95°C for 90 min. The mixture was
cooled to room temperature and the absorbance of the solution was
measured at 695 nm against blank. Based on the reading the percentage
scavenging activity was calculated (Prieto et al. 1999).