Methods
For our study we collected samples at three locations along both the Maple River and the Carp Lake River. The two locations we examined were the Hidden River Golf and Casting Club, located northeast of Brutus in Cheboygan County, Michigan and the Wilderness Golf Course located northeast of Bliss in Emmet County, Michigan. The Hidden River Golf and Casting Club is located directly on the Maple River while the Wilderness Golf course is several hundred meters away from the Carp Lake River. Both sites are located in temperate forest with the dominant tree species varying between oak, aspen, maple, and pine.