Procedure:
Pipette out the 1ml of each extracted sample in to a 25ml of
volumetric flask and add 2ml of freshly prepared anthrone
reagent in each volumetric flask and finally make up the
volume up to the mark with distilled water. Reference was
prepared by taking 2ml of anthrone reagent in a 25ml of
volumetric flask and make up the volume up to the mark
with distilled water. To the above prepared samples
wavelength was check in UV-visible spectrophotometry
and wavelength was set at 750nm at that wavelength the
developed colour absorbances were noted for the above
mentioned samples5.