Thus,
an aliquot of sperm suspension (50–100 ll) is layered
under 2 ml of capacitation medium in several tubes at
38.5°C and after 0.5–1 h the top medium (0.4–1 ml) is
collected from each tube. The pooled medium containing
highly motile spermatozoa is centrifugated
(200 9 g) for 10 min.