For TEM, the cell pellets were mixed 1:1 with molten 2% low gelling temperature agarose (Type VII, A-4018, Sigma), which was solidified by chilling, then chopped into small pieces (approximately 1 mm3).
For TEM, the cell pellets were mixed 1:1 with molten 2% lowgelling temperature agarose (Type VII, A-4018, Sigma), which wassolidified by chilling, then chopped into small pieces (approximately1 mm3).