Vitamin E (fat soluble vitamin) was analyzed by HPLC with
Diode Array and Fluorescent detector. Sample (5 g) was
weighed into a 250 ml flask. Pyrogallol acid was added as an
antioxidant followed by the addition of 40 ml of 95% ethanol.
The solution was then saponified with 10 ml of 50% potassium
hydroxide (KOH). Glacial acetic acid (10 ml) was added in
order to neutralize the KOH (DeVries and Silvera, 2002).
Vitamin E was extracted into a mixture of tetrahydrofuran
(THF) and ethanol (1:1) solution. The extract was filtered using
a 0.45 lm membrane filter into a HPLC vial for analysis.