Amplification products were checked by agarose gel electrophoresis (1% w/v), 7.5 x 10 cm (W x L) gel size, in 0.5 x TBE buffer, at a constant voltage of 100 V for 45 min; stained with 0.5 ug/mL ethidium bromide (Ludwig Biotecnologia) using 2 uL of 100 bp ladder (Ludwig Biotecnologia) as the molecular mass ladder; and visualized under ultraviolet light using a Gel Doc L-Pix image system (Loccus Biotecnologia, Brazil).