The peak of germ tube production by C. albicans ap-peared between 1.5 to 6.0 hr observed in different growth media and at different temperature and pH values and it gradually decreases after 6.0 hr. However, it was also observed in most of the cases that after decrease in % of CFGT, it starts to increase after 10.5 hr. The reason be-hind may be that the effect of growth medium, tempera-ture and pH that should be determined at regular intervals in 12 hr a day [39] is not done so as it is not possible, therefore, the experiment was splitted in to two days and observations were taken at regular intervals in 6 hr a day. Therefore, fluctuations in percentage of cells forming germ tube may be seen most of the time.