Sixty-one healthy, active people (23 men, 38 women; age = 19.99 ± 8.01 years, height = 169.42 ± 23.62 cm, mass = 71.53 ± 36.77 kg) volunteered to participate in this study. People were included if they had no history of leg trauma within the year before the study. We considered the volunteers to be healthy and active if they exercised a minimum of 3 days each week for 30 consecutive minutes per day. Leg trauma was defined as injury to the knee or the ankle. People who had diabetes or were smokers were excluded from the study because these conditions can negatively affect peripheral circulation. All participants provided written informed consent, and the study was approved by the Institutional Review Board for the Protection of Human Subjects at Indiana University.