The aim of this study was to describe behaviour, kinetics, time courses and
limitations of the six different fully automated spectrometric methods - DPPH, TEAC,
FRAP, DMPD, Free Radicals and Blue CrO5. Absorption curves were measured and
absorbance maxima were found. All methods were calibrated using the standard compounds
OPEN ACCESS
Molecules 2010, 15
8619
Trolox® and/or gallic acid. Calibration curves were determined (relative standard deviation
was within the range from 1.5 to 2.5 %). The obtained characteristics were compared and
discussed. Moreover, the data obtained were applied to optimize and to automate all
mentioned protocols. Automatic analyzer allowed us to analyse simultaneously larger set of
samples, to decrease the measurement time, to eliminate the errors and to provide data of
higher quality in comparison to manual analysis. The total time of analysis for one sample
was decreased to 10 min for all six methods. In contrary, the total time of manual
spectrometric determination was approximately 120 min. The obtained data provided good
correlations between studied methods (R = 0.97 – 0.99).