Nowadays, consumption of green leafy vegetables has been increasing, especially as a counterbalance of the
growing number of degenerative diseases. Several bioactive compounds in vegetables are including vitamins,
minerals, antioxidants, as well as the pigments (chlorophylls and carotenoids). In the living plants, chlorophylls play
an important role as primary photosynthetic pigment to capture light energy from the sun. Composed together with
carotenoids (the accessory photosynthetic pigments) in pigment-protein complexes, it exhibits colour appearance
which is specific for each plant leaf and even used as parameter of maturity, quality, and freshness of food crops1,2.
The colour is extremely important because it defines the appearance of the vegetables and influences consumer
choice3. However, this minimally processed vegetables are most kept under low temperature preservation (usually
4 °C to 10 °C, with 95 % to 100 % relative humidity) and sold within one week. During that period, the
physiological processes occur and particularly cause loss of colour due to degradation of leaf pigments or tissue
browning. Hence the quality and freshness of commercially sold vegetable can be monitored by measuring itschlorophyll contents4