2. Methods
2.1. Materials
Corncob was obtained from a local farm (Tianjin, China). It was
premilled and screened, and the fraction between 20 and 80
meshes was used for experiments. All chemicals used were of analytical
grade. Cellulase (GC220, 242 FPU/mL) was purchased from
Genencor International (Palo Alto, CA). Commercial ethanol instant
active dry yeast (S. cerevisiae) was obtained from Angel Yeast Co.,
Ltd., Wuhan, China.