The study was conducted to develop a high-performance liquid chromatographic (HPLC) method to quantify casein genetic variants (as2-, b-, and j-casein) in milk of homozygous individuals of Girgentana goat breed. For calibration experiments, pure genetic variants were extracted from individual milk samples of animals with known genotypes. The described HPLC approach was precise, accurate and highly suitable for quantification of goat casein genetic variants of homozygous individuals. The amount