The sponge mixture was further mixed into the main ingredients of the dough in a heavy-duty mixer (Kitchen Aid-KSM 900, USA) until a smooth and elastic dough formed. The dough was then allowed to rest in the proofer (Bakbar E81, New Zealand) at a temperature of 37 °C and 85% relative humidity (RH) for 40 min. The dough was then moulded manually and placed into an aluminium pan and proofed for another 1 h. It was then baked at 180 °C for 20 min in an oven (commercial turbofan Bakbar E32, New Zealand). The baked loaf was cooled at the ambient temperature for 1 h prior to packing in polyethylene bags. All loaves were stored at room temperature before the analysis.