2.10. Thiobarbituric acid value
Twenty grams of meat is blended with 50 ml of 20% trichloroacetic
acid (TCA) for 2 min. The blender content was rinsed with 50 ml of
water, mixed together, and filtered through a Whatman # 1 filter.
Subsequently, 5 ml aliquot of the TCA extract was mixed with 5 ml of
0.01 M 2-thiobarbituric acid and held at 100 °C for 1 h. The absorbance
of pink color solution was measured at 532 nm using a UV/vis spectrophotometer.
TBA was reported as mg of malonaldehyde/kg of the sample
(Strange, Benedict, Smith, & Swift, 1977).