Due to the disadvantages outlined above, saponification was
omitted from the final extraction protocol of the targeted lipophilic
antioxidants, although the tocochromanol content may be underestimated.
Briefly, in the optimized extraction method nonsaponified
and finely ground wheat samples were subjected to
three consecutive extractions with acetone, using an Ultra-Turrax
homogenization device, and extracts were combined for analysis. A
more detailed description is provided in Section 2.4.