The pretreated sugarcane leaves (pellet) were suspended in 0.5 M citrate buffer (pH 5.0) to the same volume as the separated pretreatment hydrolysate. Cellulase GC220 (71.3 FPU/ml; Genecor
International, Inc., USA) was added at 10 FPU/g (DS) and incubated at 40 C with shaking (125 rpm) for 72 h