Production of enzyme was done in Zobell marine broth. A 5% inoculum was added to Zobell broth from an overnight culture (OD 2.1 at 660 nm) and incubated for 48 h at 37°C (100 rpm); the culture was drawn after incubation and centrifuged at 10,000 rpm for 10 min at 4°C. This collected culture supernatant was used for further analysis