Sperm concentration was determined using a
Thoma-Zeiss counting cell chamber (Marienfield,
Germany). For the morphological analyses, an aliquot
from each ejaculate (20 ml) was fixed with 180 ml of a
solution of glutaraldehyde 0.2% (Electron Microscopy
Science, Washington) in Dulbecco’s Phosphate Buffered
Saline (DPBS) [16].