Yogurt is one of the most important and common dairy products that is more acceptable due
to its sensory properties and health-related values. Now most of the yogurt in Iran is high-fat yogurt and
due to the saturated fat and cholesterol and the relation of this kind of fat with some diseases, the
consumption of yogurt got problematic. Substitution of the fat of milk with healthy vegetable oil is one of
the strategies of producing high fat yogurt by keeping the sensory, tissue properties and improving
nutritional properties. In the current study, the effect of substitution of milk fat with refined sesame oil on
sensory, physicochemical and rheological properties of high-fat yogurt is studied. After the thickening of
the initial milk by adding 2% non-fat dry milk, the milk fat content was adjusted by mixing the cream and
sesame oil. The results of the study showed that acidity changes and PH didn’t have correlation with
sesame oil ratio in each sample but the samples with more sesame oil had high PH. The fat percent of the
samples at the end of the storage period was partially more than the beginning of the period. Thus, this
study showed that in case of formulation modification, we can use sesame oil in production of high-fat
yogurt as the substitute of a part of milk fat.